Produced:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Virus:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Recombinant:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Expressing:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Lysis:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Luciferase:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Cloning:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
SYBR Green Assay:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Polymerase Chain Reaction:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
CCK-8 Assay:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
Cell Counting:Article Title: Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA antibody produced in rabbit Sigma-Aldrich Cat#H6908; RRID: AB_260070 Ebola virus Glycoprotein Antibody Sino Biological Cat#40442-T62 Anti-VSV-M antibody Kerafast Cat#EB0011; RRID: AB_2734773 Anti-β-actin antibody Sigma-Aldrich Cat#A5441; RRID: AB_476744 IRDye® 680RD Goat anti-Rabbit IgG Li-COR Cat#926-68071; RRID: AB_10956166 IRDye® 800CW Goat anti-Mouse IgG Li-COR Cat#926-32210; RRID: AB_621842 mAb114 Gaudinski et al. 35 N/A 1A10-Fc This paper N/A BC2-Fc This paper N/A BA2-Fc This paper N/A REGN3470 MedChemExpress Cat#HY-P99343; RRID: AB_3695157 REGN3471 MedChemExpress Cat#HY-P99764; RRID: AB_3695448 Chemicals, peptides, and recombinant proteins Nhe I enzyme Thermo Fisher Scientific Cat#FD0974 Xho I enzyme Thermo Fisher Scientific Cat#FD0694 PrimeSTAR GXL DNA Polymerase Takara Cat#R050A TransIT-LT1 Mirus Cat#MIR2300 DMEM, high glucose Thermo Fisher Scientific Cat#11995073 Expi293 expression medium Thermo Fisher Scientific Cat#A1435101 Fetal Bovine Serum Gibco Cat#10099-141C D-Luciferin potassium salt Beyotime Cat#ST196 Glo lysis buffer Promega Cat#E2661 LipofectamineTM 2000 Reagent Thermo Fisher Scientific Cat#11668019 Sucrose Sigma-Aldrich Cat#V900116 PBS Beyotime Cat#ST476 TWEEN 20 Sigma-Aldrich Cat#P1379 NaCl Sigma-Aldrich Cat#S5150 KCl Sigma-Aldrich Cat#V900068 NP40 Beyotime Cat#P0013F Na2HPO4 Sigma-Aldrich Cat#V900061 Thermolysin Sigma-Aldrich Cat#P1512 CA074 MedChemExpress Cat#HY-103350 Z-FY(t-Bu)-dmk MedChemExpress Cat#HY-138208 3.47 MedChemExpress Cat#HY-W395779 U18666A MedChemExpress Cat#HY-107433 Tubeimoside III MedChemExpress Cat#HY-N2542 DMSO Sigma-Aldrich Cat#D5879 Critical commercial assays Dual-luciferase assay kit Promega Cat#E2920 Luciferase assay system kit Promega Cat#E1500 ClonExpress II One Step Cloning Kit Vazyme Cat#C112 SYBR Green PCR kit Takara Cat#RR091A CCK-8 Cell Counting Kit Vazyme Cat#A311 (Continued on next page) ll OPEN ACCESS Cell 189, 1–14.e1–e7, February 5, 2026 e1 Please cite this article in press as: Fan et al., Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018–2020 epidemic, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.022 Article
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